Abstract
Analysis of severe acute respiratory syndrome coronavirus (SCoV) by either sucrose gradient equilibrium centrifugation or a virus capture assay using an anti-SCoV S protein antibody demonstrated that the SCoV 6 protein, which is one of the accessory proteins of SCoV, was incorporated into virus particles. Coexpression of the SCoV S, M, E, and 6 proteins was sufficient for incorporation of the 6 protein into virus-like particles. Cells transfected with plasmid expressing the 6 protein released SCoV 6 protein; however, infected cells released SCoV 6 protein only in association with SCoV particles.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Centrifugation, Density Gradient
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Chlorocebus aethiops
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Severe acute respiratory syndrome-related coronavirus / chemistry
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Severe acute respiratory syndrome-related coronavirus / isolation & purification
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Severe acute respiratory syndrome-related coronavirus / metabolism*
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Vero Cells
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Viral Proteins / analysis
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Viral Proteins / metabolism*
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Virion / chemistry
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Virion / isolation & purification
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Virion / metabolism*
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Virosomes / chemistry
Substances
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Viral Proteins
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Virosomes
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protein 6, SARS virus