Regioselective and enzymatic production of gamma-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

Yuichiro Iwasaki; Kuniki Kino; Hiroyuki Nishide; Kohtaro Kirimura

doi:10.1007/s10529-007-9309-6

Regioselective and enzymatic production of gamma-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

Biotechnol Lett. 2007 May;29(5):819-22. doi: 10.1007/s10529-007-9309-6. Epub 2007 Feb 15.

Authors

Yuichiro Iwasaki¹, Kuniki Kino, Hiroyuki Nishide, Kohtaro Kirimura

Affiliation

¹ Department of Applied Chemistry, School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan.

PMID: 17415666
DOI: 10.1007/s10529-007-9309-6

Abstract

A recombinant Escherichia coli, expressing the rdc gene, which encodes a gamma-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to gamma-resorcylic acid with a 44% (mol/mol) yield at 30 degrees C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium tumefaciens / enzymology*
Carboxy-Lyases / genetics*
Catalysis
Escherichia coli / cytology*
Escherichia coli / genetics*
Gene Expression* / drug effects
Hydroxybenzoates / chemistry
Hydroxybenzoates / metabolism*
Resorcinols / chemistry
Resorcinols / metabolism*
Resorcinols / pharmacology
Stereoisomerism

Substances

Hydroxybenzoates
Resorcinols
Carboxy-Lyases
gamma-resorcylic acid decarboxylase, Rhizobium radiobacter
gamma-resorcylic acid
resorcinol