Objective: To observe the effect of warm ischemia-reperfusion injury (WIRI) on the genes involved in the cell cycle in liver and investigate the molecular mechanism of WIRI.
Methods: Twenty-four Sprague-Dawley male rats were divided into experiment groups and sham operation group respectively. According to the dynamic characteristics, the genes involved in the cell cycle were submitted for multivariate cluster and self-organized map analysis. Total RNA was extracted for the microarray analysis and subsequent real-time quantitative PCR.
Results: There were 86, 410, 234 up-regulated genes and 136, 312, 653 down-regulated genes over 2 - 4 times in the 0 h, 1 h and 24 h group respectively, most of which such as Gadd45a, Egr1, Hsp70 were involved in the cell cycle checkpoint, energy metabolism, signal transduction, immune-associated procedure, and so on. In addition, the results of microarray analysis were confirmed by real-time quantitative PCR.
Conclusions: Some genes related with cell cycle and regeneration were activated in the early stage of WIRI. Gadd45a, Egr1 and Hsp70 may be new targets to cure WIRI.