A fundamental issue in mechanotransduction is to determine pathways of stress propagation in the cytoplasm. We describe a recently developed synchronous detection approach that can be used to map nanoscale distortions of cytoskeletal elements and nuclear structures in living individual cells using green fluorescent protein technology and 3D magnetic twisting cytometry. This approach could be combined with single-cell biochemical and biological assays to help elucidate mechanisms of mechanotransduction.