[Combined application of multiplex reverse transcription-polymerase chain reaction and karyotype analysis to detection of clonal chromosomal aberrations in acute myeloid leukemia]

Ai Zheng. 2007 Sep;26(9):1029-33.
[Article in Chinese]

Abstract

Background & objective: Cytogenetic analysis plays a critical role in the diagnosis and prognosis evaluation of leukemia, but karyotype analysis is time-consuming and difficult to yield sufficient metaphases; while polymerase chain reaction (PCR) is sensitive and efficient. This study was to investigate combined application of multiplex reverse transcription-polymerase chain reaction (RT-PCR) and karyotype analysis to the detection of clonal chromosomal aberrations in acute myeloid leukemia (AML), and explore the expression and distribution of fusion genes among the subtypes of AML.

Methods: Sixty AML patients were examined by multiplex RT-PCR. Cytogenetic data were obtained from 37 of them by R or G banding techniques.

Results: Fusion genes, including AML1/ETO, PML/RARalpha, CBFbeta/MYH11, MLL gene rearrangements (that is, MLL/AF6, MLL/AF9, MLL/AF10, and MLL/MLL), DEK/CAN, TEL/PDGFR, and AML1/MDS1 (EVI-1), were detected in 28 (46.7%) patients by multiplex RT-PCR. In the 37 patients who received karyotype analysis, data were available in 30 patients and cytogenetic aberrations were detected in only 14 (46.7%) of them. The detection rate of clonal chromosomal aberrations was enhanced to 59.5% by combined application of multiplex RT-PCR and karyotype analysis.

Conclusion: Multiplex RT-PCR combined with karyotype analysis can improve the detection rate of clonal chromosomal aberrations in AML.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Child, Preschool
  • Chromosome Aberrations*
  • Chromosome Banding
  • Female
  • Humans
  • Karyotyping / methods
  • Leukemia, Myeloid, Acute / classification
  • Leukemia, Myeloid, Acute / genetics*
  • Male
  • Middle Aged
  • Oncogene Proteins, Fusion / analysis
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Young Adult

Substances

  • Oncogene Proteins, Fusion