A method was developed for the investigation of the contents and the speciation of zinc in bunge pricklyash seed (BPS) in different habitats by atomic absorption spectrum (AAS). By using 0.45 microm filter membrane and chloroform, four different species of the decoction of BPS, named the suspended, the soluble, the inorganic and organic species, were obtained, and the segregation analysis methods for those species were developed. The octanol-water system was proposed to simulate the distribution of decocted zinc in the stomach and the intestine of the human body. The results showed that the decocted zinc was the main content of the total zinc, and the contents of zinc were different in different habitats, but the percentages of decocted zinc and the organism zinc were similar. The recovery for the method was 97.5%-104.0% and the relative standard deviation was less than 2.36%. The results showed that the sensitivity and reproducibility of this method were excellent.