Characterization of new staphylococcal cassette chromosome mec (SCCmec) and topoisomerase genes in fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius

J Clin Microbiol. 2008 May;46(5):1818-23. doi: 10.1128/JCM.02255-07. Epub 2008 Feb 27.

Abstract

Fluoroquinolone- and methicillin-resistant Staphylococcus pseudintermedius isolates harbor two new staphylococcal cassette chromosome mec (SCCmec) elements that belong to class A, allotype 3 (SCCmec II-III), and to the new allotype 5 (SCCmec VII). Analysis of the complete nucleotide sequences of the topoisomerase loci gyrB/gyrA and grlB/grlA revealed mutations involved in fluoroquinolone resistance.

MeSH terms

  • Amino Acid Substitution / genetics
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics
  • Chromosomes, Bacterial*
  • DNA Topoisomerases / genetics
  • Dog Diseases / microbiology
  • Dogs
  • Drug Resistance, Bacterial / genetics*
  • Fluoroquinolones / pharmacology*
  • Genes, Bacterial*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Mutation, Missense
  • Sequence Analysis, DNA
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / veterinary
  • Staphylococcus / drug effects*
  • Staphylococcus / genetics*
  • Staphylococcus / isolation & purification

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Fluoroquinolones
  • DNA Topoisomerases

Associated data

  • GENBANK/AM262967
  • GENBANK/AM262968
  • GENBANK/AM262969
  • GENBANK/AM262970
  • GENBANK/AM262971
  • GENBANK/AM262972
  • GENBANK/AM904731
  • GENBANK/AM904732