L-Ribulose production from L-arabinose by an L-arabinose isomerase mutant from Geobacillus thermodenitrificans

Biotechnol Lett. 2008 Oct;30(10):1789-93. doi: 10.1007/s10529-008-9746-x. Epub 2008 May 30.

Abstract

Geobacillus thermodenitrificans, with a double-site mutation in L-arabinose isomerase, produced 95 g L-ribulose l(-1 ) from 500 g L-arabinose l(-1) under optimum conditions of pH 8, 70 degrees C, and 10 units enzyme ml(-1) with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75 degrees C were 35 and 4.5 h, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases / genetics
  • Aldose-Ketose Isomerases / metabolism*
  • Arabinose / metabolism*
  • Enzyme Stability
  • Geobacter / enzymology*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Mutation*
  • Pentoses / biosynthesis*
  • Temperature

Substances

  • Pentoses
  • ribulose
  • Arabinose
  • Aldose-Ketose Isomerases
  • L-arabinose isomerase