Abstract
Geobacillus thermodenitrificans, with a double-site mutation in L-arabinose isomerase, produced 95 g L-ribulose l(-1 ) from 500 g L-arabinose l(-1) under optimum conditions of pH 8, 70 degrees C, and 10 units enzyme ml(-1) with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75 degrees C were 35 and 4.5 h, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aldose-Ketose Isomerases / genetics
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Aldose-Ketose Isomerases / metabolism*
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Arabinose / metabolism*
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Enzyme Stability
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Geobacter / enzymology*
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Hydrogen-Ion Concentration
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Kinetics
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Mutation*
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Pentoses / biosynthesis*
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Temperature
Substances
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Pentoses
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ribulose
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Arabinose
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Aldose-Ketose Isomerases
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L-arabinose isomerase