Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet

Nucleic Acids Res. 2008 Nov;36(20):6548-57. doi: 10.1093/nar/gkn703. Epub 2008 Oct 16.

Abstract

Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anticodon / chemistry*
  • Base Sequence
  • Codon / metabolism
  • Cytidine / analogs & derivatives*
  • Cytidine / chemistry
  • Humans
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Protein Biosynthesis*
  • RNA / chemistry*
  • RNA, Mitochondrial
  • RNA, Transfer, Met / chemical synthesis
  • RNA, Transfer, Met / chemistry*
  • RNA, Transfer, Met / metabolism
  • Thermodynamics

Substances

  • Anticodon
  • Codon
  • RNA, Mitochondrial
  • RNA, Transfer, Met
  • 5-formylcytidine
  • Cytidine
  • RNA