Abstract
We examined the in vitro effects of imatinib (Novartis Pharma AG, Basel, Switzerland) as a possible inhibitor of PDGFR pathway on cells derived from a recurrence of a pleural malignant solitary fibrous tumor (SFT). Primary cell culture was characterised by immunofluorescence. SFT-derived cells were treated with imatinib at different time points. Western blotting for PDGFR-beta, phospho-PDGFR-beta or smooth muscle actin (SMA) was performed before and after 96 h of treatment with imatinib. SFT-derived cells treated with imatinib for 96 h showed a dose dependent decrease of Ki67 expression. Results were confirmed by growth curve. Western blotting showed that PDGFR-beta was highly expressed and phosphorylated in SFT-derived cells and imatinib treatment reduced PDGFR-beta phosphorylation and SMA expression. With the limit of experimental findings, our results support a possible future application of imatinib as a candidate molecule in the target therapy of malignant SFTs over-expressing wild-type PDGFR.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Antineoplastic Agents / pharmacology*
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Antineoplastic Combined Chemotherapy Protocols / therapeutic use
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Benzamides
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Blotting, Western
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Cell Proliferation / drug effects*
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Cells, Cultured
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Cisplatin / administration & dosage
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Female
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Fluorescent Antibody Technique
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Fluorouracil / administration & dosage
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Humans
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Imatinib Mesylate
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In Vitro Techniques
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Neoplasm Recurrence, Local / metabolism
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Neoplasm Recurrence, Local / pathology
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Piperazines / pharmacology*
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Pneumonectomy
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Pyrimidines / pharmacology*
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Radiotherapy
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Receptor, Platelet-Derived Growth Factor alpha / biosynthesis
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Receptor, Platelet-Derived Growth Factor beta / biosynthesis
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Receptor, Platelet-Derived Growth Factor beta / drug effects*
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Solitary Fibrous Tumor, Pleural / metabolism*
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Solitary Fibrous Tumor, Pleural / pathology
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Solitary Fibrous Tumor, Pleural / therapy
Substances
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Antineoplastic Agents
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Benzamides
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Piperazines
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Pyrimidines
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Imatinib Mesylate
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Receptor, Platelet-Derived Growth Factor alpha
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Receptor, Platelet-Derived Growth Factor beta
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Cisplatin
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Fluorouracil