Abstract
To explore the best prime-boost regimen and evaluate the T-cellular response memory against HCV, we constructed two DNA vaccine candidates (pVRC-CE1E2 and pAAV-CE1E2) and two recombinant viruses (rTTV-E1E2 and rAAV-E1E2) and then assessed the immune response to different prime-boost patterns in BALB/c mice. The rTTV-E1E2 boosted the immune response to HCV DNA vaccine prime significantly, and the inverted terminal repeat sequence harboring DNA construct PAAV-CE1E2 was the best prime agent in this study. Our study provides new information for both the prime-boost regimen and long-term T-cell response for HCV vaccine development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Dependovirus / genetics
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Female
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Hepacivirus / genetics
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Hepacivirus / immunology*
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Hepatitis Antibodies / blood
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Hepatitis Antibodies / immunology
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Immunization, Secondary
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Immunologic Memory / immunology
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Inverted Repeat Sequences
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Mice
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Mice, Inbred BALB C
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Plasmids
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T-Lymphocytes / immunology*
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T-Lymphocytes / virology
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / immunology*
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Vaccinia virus / genetics*
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Viral Core Proteins / genetics
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Viral Core Proteins / immunology
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology
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Viral Hepatitis Vaccines / administration & dosage
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Viral Hepatitis Vaccines / immunology*
Substances
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E1 protein, Hepatitis C virus
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Hepatitis Antibodies
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Vaccines, DNA
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Viral Core Proteins
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Viral Envelope Proteins
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Viral Hepatitis Vaccines
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nucleocapsid protein, Hepatitis C virus
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glycoprotein E2, Hepatitis C virus