Abstract
Poststatin, a new inhibitor of prolyl endopeptidase (PEP) was discovered in the fermentation broth of Streptomyces viridochromogenes MH534-30F3. It was purified by Diaion HP-20, Sephadex LH-20 and YMC-gel (ODS-A) column chromatography and then isolated as a colorless powder. Poststatin has the molecular formula C26H47N5O7. The IC50 value of poststatin against the PEP of partially purified porcine kidney was 0.03 microgram/ml. It has low acute toxicity. No deaths occured after iv injection of 250 mg/kg of this agent to mice.
MeSH terms
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Amino Acid Sequence
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Animals
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Cathepsin D / antagonists & inhibitors
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Chemical Phenomena
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Chemistry
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Endopeptidases / metabolism*
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Mice
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Molecular Sequence Data
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Oligopeptides / isolation & purification*
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Oligopeptides / pharmacology
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Prolyl Oligopeptidases
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Serine Endopeptidases*
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Serine Proteinase Inhibitors / isolation & purification*
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Serine Proteinase Inhibitors / pharmacology
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Streptomyces / chemistry*
Substances
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Oligopeptides
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Serine Proteinase Inhibitors
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poststatin
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Endopeptidases
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Serine Endopeptidases
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Prolyl Oligopeptidases
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Cathepsin D