Abstract
Pig edema disease is a bacterial disease caused by Shiga toxin 2e-producing Escherichia coli belonging mainly to serotypes O138, O139, and O141. The B subunit of Shiga toxin 2e (Stx2eB) is a candidate protein for use in a vaccine against edema disease. We produced this protein in transgenic lettuce (Lactuca sativa), an edible plant that can be cultivated in a factory setting. In a transient expression system, we found that NtADH 5'-untranslated region (5'-UTR) functions as a translational enhancer in lettuce cells, and that Stx2eB accumulates most efficiently in the endoplasmic reticulum (ER) of lettuce cells. Stx2eB was produced in stable transgenic lettuce plants expressing a modified Stx2eB gene fused with the NtADH 5'-UTR and sequence encoding ER localization signals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Untranslated Regions / genetics
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Alcohol Dehydrogenase / genetics
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Animals
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Bacterial Vaccines / biosynthesis
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Bacterial Vaccines / genetics
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Bacterial Vaccines / immunology
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Bacterial Vaccines / metabolism*
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Edema / immunology*
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Edema / prevention & control
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Endoplasmic Reticulum / metabolism
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Escherichia coli Infections / immunology
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Escherichia coli Infections / prevention & control
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Escherichia coli Infections / veterinary
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Lactuca / genetics*
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Lactuca / metabolism*
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Nicotiana / enzymology
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Plants, Genetically Modified
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Protein Biosynthesis
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Protein Engineering / methods*
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Protein Transport
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Reproducibility of Results
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Shiga Toxin 2 / biosynthesis*
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Shiga Toxin 2 / genetics
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Shiga Toxin 2 / immunology*
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Shiga Toxin 2 / metabolism
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Sus scrofa / immunology
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Sus scrofa / microbiology
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Swine Diseases / immunology
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Swine Diseases / prevention & control
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Vaccines, Edible / biosynthesis
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Vaccines, Edible / genetics
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Vaccines, Edible / immunology
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Vaccines, Edible / metabolism
Substances
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5' Untranslated Regions
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Bacterial Vaccines
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Shiga Toxin 2
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Vaccines, Edible
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Alcohol Dehydrogenase