Purification of Arabidopsis argonaute complexes and associated small RNAs

Yijun Qi; Shijun Mi

doi:10.1007/978-1-60327-005-2_16

Purification of Arabidopsis argonaute complexes and associated small RNAs

Methods Mol Biol. 2010:592:243-54. doi: 10.1007/978-1-60327-005-2_16.

Authors

Yijun Qi¹, Shijun Mi

Affiliation

¹ National Institute of Biological Sciences, Beijing, China.

PMID: 19802600
DOI: 10.1007/978-1-60327-005-2_16

Abstract

Argonaute (AGO) proteins recruit small RNAs to form effector complexes of RNA interference (RNAi), collectively termed RNA-induced silencing complexes (RISCs). Here, we describe detailed protocols for the purification of AGO complexes and their associated small RNAs, using Arabidopsis AGO1 as an example.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis Proteins / chemistry
Arabidopsis Proteins / metabolism
Argonaute Proteins
Blotting, Western
DNA Methylation
RNA, Small Interfering / chemistry*
RNA, Small Interfering / metabolism*
RNA-Induced Silencing Complex / chemistry
RNA-Induced Silencing Complex / isolation & purification*

Substances

AGO1 protein, Arabidopsis
AGO4 protein, Arabidopsis
AGO6 protein, Arabidopsis
Arabidopsis Proteins
Argonaute Proteins
RNA, Small Interfering
RNA-Induced Silencing Complex