Localization of the dihydrolipoamide branched-chain transacylase gene (DBT) of the human branched-chain keto acid dehydrogenase complex to chromosome 1

Cytogenet Cell Genet. 1991;56(1):33-5. doi: 10.1159/000133041.

Abstract

The gene coding for the transacylase subunit (DBT) of the human branched-chain keto acid dehydrogenase complex was localized to chromosome 1 by probing panels of human x mouse chromosome hybrids with an E2 cDNA amplified by the polymerase chain reaction. Additional data with two hybrids containing chromosome 1 fragments suggest that the DBT gene is located on the short arm (1pter----p21) of the chromosome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
  • Acyltransferases / genetics*
  • Acyltransferases / metabolism
  • Animals
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1*
  • DNA Probes
  • Humans
  • Hybrid Cells
  • Ketone Oxidoreductases / genetics*
  • Ketone Oxidoreductases / metabolism
  • Mice
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Multienzyme Complexes / metabolism
  • Polymerase Chain Reaction

Substances

  • DNA Probes
  • Multienzyme Complexes
  • Ketone Oxidoreductases
  • 3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
  • Acyltransferases
  • dihydrolipoamide acyltransferase