Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells

Tadatoshi Sugio; Hideyuki Matsuura; Takeshi Matsui; Manabu Matsunaga; Tatsuya Nosho; Shigehiko Kanaya; Atsuhiko Shinmyo; Ko Kato

doi:10.1016/j.jbiosc.2009.07.009

Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells

J Biosci Bioeng. 2010 Feb;109(2):170-3. doi: 10.1016/j.jbiosc.2009.07.009. Epub 2009 Aug 14.

Authors

Tadatoshi Sugio¹, Hideyuki Matsuura, Takeshi Matsui, Manabu Matsunaga, Tatsuya Nosho, Shigehiko Kanaya, Atsuhiko Shinmyo, Ko Kato

Affiliation

¹ Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara, Japan.

PMID: 20129102
DOI: 10.1016/j.jbiosc.2009.07.009

Abstract

The sequence context around the AUG initiation codon strongly contributes to the translation initiation step in mammalian and plant cells. Here, we investigated the effect of the three nucleotides immediately upstream of the initiating AUG (positions -3 to -1) on the translation efficiency of a reporter gene, beta-glucuronidase, in dicotyledonous and monocotyledonous plant cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / genetics
Arabidopsis / metabolism
Codon, Initiator / genetics*
Glucuronidase / genetics
Glucuronidase / metabolism
Magnoliopsida / genetics
Magnoliopsida / metabolism*
Oryza / genetics
Oryza / metabolism
Protein Biosynthesis / genetics*
Protoplasts / metabolism

Substances

Codon, Initiator
Glucuronidase