Detection, quantification, and characterisation of HIV/SIV

Methods Mol Biol. 2011:665:133-60. doi: 10.1007/978-1-60761-817-1_9.

Abstract

Selected techniques for the detection, quantification, and characterisation of HIV1, HIV2, and SIV, as applied to diagnostic and research purposes, are described. Representative nucleic acid testing protocols including nested PCR, RT-PCR, and quantitative real-time PCR, as well as protocols based on virus infectivity, are presented.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • HIV Infections / diagnosis*
  • HIV Infections / virology
  • HIV* / genetics
  • HIV* / isolation & purification
  • HIV* / physiology
  • HIV-1 / genetics
  • HIV-1 / isolation & purification
  • HIV-2 / genetics
  • HIV-2 / isolation & purification
  • Humans
  • Polymerase Chain Reaction / methods*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Simian Acquired Immunodeficiency Syndrome / diagnosis*
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / isolation & purification*