Abstract
T cell lines with a novel phenotype (CD3+ TCR-alpha/beta+ CD4- CD8-) were developed from the peripheral blood of a patient with a combined immunodeficiency and tissue injury resembling graft-vs-host disease. One of these IL-2-dependent T cell lines demonstrated non-MHC-restricted cytolytic function against tumor targets, syngeneic and allogeneic fibroblasts, and PHA blasts from allogeneic donors. The other cell line only became cytotoxic in the presence of lectin or anti-CD3 antibody. The two cell lines also differed in their expression of the T-200 gene products CD45RO (gp180) and CD45RA (gp220). Both cell lines produced tumor necrosis factor-alpha and -beta and IFN-gamma activity when activated with mitogens or PMA and IL-1. The in vitro functions of these T-cell lines suggest a potential role for alpha/beta double-negative T lymphocytes in tissue injury resembling graft-vs-host disease.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD / analysis*
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Antigens, Differentiation, T-Lymphocyte / analysis
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Antigens, Differentiation, T-Lymphocyte / genetics
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Blotting, Northern
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CD3 Complex
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CD4 Antigens / analysis
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CD4 Antigens / genetics
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CD8 Antigens
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Cytotoxicity, Immunologic
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Flow Cytometry
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Humans
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Immunity, Cellular
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Immunologic Deficiency Syndromes / immunology*
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Infant
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Interferon-gamma / biosynthesis
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Lymphocyte Activation
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Lymphokines / biosynthesis*
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Major Histocompatibility Complex
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Male
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Receptors, Antigen, T-Cell / analysis
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Receptors, Antigen, T-Cell / classification*
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T-Lymphocytes, Cytotoxic / immunology*
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Tumor Necrosis Factor-alpha / biosynthesis*
Substances
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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CD3 Complex
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CD4 Antigens
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CD8 Antigens
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Lymphokines
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Receptors, Antigen, T-Cell
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Tumor Necrosis Factor-alpha
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Interferon-gamma