Abstract
The vpr gene of human immunodeficiency virus type 1 (HIV-1) is one of the seven accessory genes that are believed to have roles in the virus replication cycle. We report here the detection of a 13 kD vpr protein in sucrose gradient-purified HIV-1. This protein was not detected in cells infected with a virus having a truncated vpr gene that lacks the potential to encode for 26 C-terminal amino acid residues. These findings raise the possibility that virion-associated vpr proteins may be involved in the early life cycle of HIV-1 replication and suggest that the C-terminal region of the vpr gene is essential for its expression.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Line
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Centrifugation, Density Gradient
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Gene Expression Regulation, Viral
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Gene Products, vpr / genetics*
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Genes, Viral
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HIV Infections / microbiology
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HIV-1 / genetics*
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HIV-1 / physiology
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Humans
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Molecular Sequence Data
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Recombinant Proteins / biosynthesis
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Sequence Homology, Nucleic Acid
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Viral Proteins / biosynthesis
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Viral Proteins / genetics*
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Virion / genetics
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Virus Replication
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vpr Gene Products, Human Immunodeficiency Virus
Substances
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Gene Products, vpr
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Recombinant Proteins
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Viral Proteins
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vpr Gene Products, Human Immunodeficiency Virus