Enzymatic assay method for measuring mizoribine levels in serum

Takahisa Hiramitsu; Hiroko Ota; Yoshihiko Watarai; Masahito Achiha; Harue Fukami; Shin-ichi Sakasegawa; Emisa Hino; Tamami Yamaguchi; Shigeru Ueda; Yoshitaka Kagimoto; Tomohiro Tamura; Kazuharu Uchida

doi:10.1016/j.jbiosc.2011.03.017

Enzymatic assay method for measuring mizoribine levels in serum

J Biosci Bioeng. 2011 Aug;112(2):205-7. doi: 10.1016/j.jbiosc.2011.03.017. Epub 2011 May 6.

Authors

Takahisa Hiramitsu¹, Hiroko Ota, Yoshihiko Watarai, Masahito Achiha, Harue Fukami, Shin-ichi Sakasegawa, Emisa Hino, Tamami Yamaguchi, Shigeru Ueda, Yoshitaka Kagimoto, Tomohiro Tamura, Kazuharu Uchida

Affiliation

¹ Nagoya Daini Red Cross Hospital, 2-9 Myoken-cho, Showa-ku, Nagoya 466-8650, Japan.

PMID: 21530390
DOI: 10.1016/j.jbiosc.2011.03.017

Abstract

A sensitive and specific method for assaying serum mizoribine levels that can be applied to general automatic clinical analyzers was developed. Regression analysis of the enzymatic assay (y) vs. the HPLC method (x) produced the following relation: y=0.964x+0.090 (n=262, Sy, x=6.37 ng/mL).

MeSH terms

Chromatography, High Pressure Liquid
Enzyme Assays / methods*
Humans
Immunosuppressive Agents / blood*
Regression Analysis
Ribonucleosides / blood*

Substances

Immunosuppressive Agents
Ribonucleosides
mizoribine