Effect of the EBNA-2 gene on the surface antigen phenotype of transfected EBV-negative B-lymphoma lines

Int J Cancer. 1990 Jan 15;45(1):77-82. doi: 10.1002/ijc.2910450115.

Abstract

Two EBV-negative B-lymphoma cell lines with different phenotypes were transfected with the Epstein-Barr virus EBNA-2 gene. The effects on the expression of 8 B-cell surface markers were analyzed by immunofluorescence methods. In one of the EBNA-2 transfected cell lines, the expression of the CR2 receptor CD21 was induced and the expression of CD23 was enhanced. The results suggest that the EBNA-2 gene is involved in the regulation of CD21 and CD23 in EBV-carrying cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Antibodies, Viral / analysis
  • Antigens, Neoplasm / genetics*
  • Antigens, Surface / genetics
  • B-Lymphocytes
  • Cell Line
  • Cosmids / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Viral / genetics
  • Genes, Viral*
  • Genetic Vectors / genetics
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Immunoblotting
  • Lymphoma / genetics*
  • Lymphoma / immunology
  • Phenotype
  • Transfection / genetics*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Neoplasm
  • Antigens, Surface