Abstract
Two EBV-negative B-lymphoma cell lines with different phenotypes were transfected with the Epstein-Barr virus EBNA-2 gene. The effects on the expression of 8 B-cell surface markers were analyzed by immunofluorescence methods. In one of the EBNA-2 transfected cell lines, the expression of the CR2 receptor CD21 was induced and the expression of CD23 was enhanced. The results suggest that the EBNA-2 gene is involved in the regulation of CD21 and CD23 in EBV-carrying cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Antibodies, Viral / analysis
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Antigens, Neoplasm / genetics*
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Antigens, Surface / genetics
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B-Lymphocytes
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Cell Line
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Cosmids / genetics
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Electrophoresis, Polyacrylamide Gel
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Fluorescent Antibody Technique
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Gene Expression Regulation, Viral / genetics
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Genes, Viral*
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Genetic Vectors / genetics
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Herpesvirus 4, Human / genetics*
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Humans
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Immunoblotting
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Lymphoma / genetics*
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Lymphoma / immunology
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Phenotype
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Transfection / genetics*
Substances
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Antibodies, Monoclonal
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Antibodies, Viral
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Antigens, Neoplasm
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Antigens, Surface