Comparison of a novel real-time PCR assay with sequence analysis, reverse hybridization, and multiplex PCR for hepatitis B virus type B and C genotyping

J Clin Microbiol. 2011 Sep;49(9):3392-4. doi: 10.1128/JCM.00543-11. Epub 2011 Jul 13.

Abstract

We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Genotype
  • Hepatitis B / virology
  • Hepatitis B virus / classification*
  • Hepatitis B virus / genetics*
  • Hepatitis B virus / isolation & purification
  • Humans
  • Molecular Typing / methods
  • Multiplex Polymerase Chain Reaction / methods*
  • Nucleic Acid Hybridization / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA / methods*
  • Virology / methods*