Abstract
We compared a novel real-time genotyping and quantitative PCR (GQ-PCR) assay, direct sequence analysis, reverse hybridization, and multiplex PCR for genotyping hepatitis B virus (HBV) in 127 HBV-infected patients. We found that GQ-PCR had the highest concordance with sequence analysis and the highest detection rate for mixed genotype detecting.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Genotype
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Hepatitis B / virology
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Hepatitis B virus / classification*
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Hepatitis B virus / genetics*
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Hepatitis B virus / isolation & purification
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Humans
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Molecular Typing / methods
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Multiplex Polymerase Chain Reaction / methods*
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Nucleic Acid Hybridization / methods*
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Real-Time Polymerase Chain Reaction / methods*
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Sequence Analysis, DNA / methods*
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Virology / methods*