Placement of running, locking stitches during the harvest and preparation of hamstring tendons can be time consuming and requires placement of suture in the most distal and proximal 2 to 3 cm of the tendons. The authors have regularly used a needleless suture loop technique to both harvest and prepare autograft gracilis and semitendinosis tendons and to tension quadrupled hamstring tendon grafts during anterior cruciate ligament reconstruction. This cost-effective technique reduces exposure to needles, the time required to harvest and prepare the grafts, and the amount of suture in the tibial tunnel, while providing adequate fixation to tension the tendons as needed.