Whole-genome metabolic network reconstruction and constraint-based modeling

Methods Enzymol. 2011:500:411-33. doi: 10.1016/B978-0-12-385118-5.00021-9.

Abstract

With the advent of modern high-throughput genomics, there is a significant need for genome-scale analysis techniques that can assist in complex systems analysis. Metabolic genome-scale network reconstructions (GENREs) paired with constraint-based modeling are an efficient method to integrate genomics, transcriptomics, and proteomics to conduct organism-specific analysis. This text explains key steps in the GENRE construction process and several methods of constraint-based modeling that can help elucidate basic life processes and development of disease treatment, bioenergy solutions, and industrial bioproduction applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Animals
  • Bayes Theorem
  • Computer Simulation*
  • Databases, Genetic
  • Gene Expression Profiling
  • Genome, Human
  • Humans
  • Metabolic Networks and Pathways / genetics*
  • Models, Biological*
  • Systems Biology