Ten years of HIV testing with fourth generation assays: the Amsterdam experience

Suzanne Jurriaans; Nicole K T Back; Katja C Wolthers

doi:10.1016/j.jcv.2011.09.021

Ten years of HIV testing with fourth generation assays: the Amsterdam experience

J Clin Virol. 2011 Dec:52 Suppl 1:S67-9. doi: 10.1016/j.jcv.2011.09.021. Epub 2011 Oct 12.

Authors

Suzanne Jurriaans¹, Nicole K T Back, Katja C Wolthers

Affiliation

¹ Department of Medical Microbiology, Laboratory of Clinical Virology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

PMID: 21995933
DOI: 10.1016/j.jcv.2011.09.021

Abstract

Serological HIV assays combining detection of HIV antigen and antibodies are referred to as fourth generation assays. Fourth generation assays were implemented in Europe for routine patient testing about 10 years ago. The Academic Medical Center is one of the main HIV treatment centers in the Netherlands and has now 10 years experience with fourth generation testing, which is summarized here.

MeSH terms

Algorithms
Blotting, Western
HIV Antibodies / analysis*
HIV Antibodies / immunology
HIV Antigens / analysis*
HIV Antigens / immunology
HIV Infections / diagnosis*
HIV Infections / epidemiology
HIV Infections / immunology
HIV Infections / virology
HIV-1 / genetics
HIV-1 / immunology
HIV-1 / pathogenicity
HIV-2 / genetics
HIV-2 / immunology
HIV-2 / pathogenicity
Humans
Immunoassay / methods
Mass Screening / methods
Netherlands / epidemiology
RNA, Viral / analysis
Serologic Tests / methods
Time Factors
Viral Load

Substances

HIV Antibodies
HIV Antigens
RNA, Viral