Biofilm accumulation on endotracheal tubes following prolonged intubation

J Laryngol Otol. 2012 Mar;126(3):267-70. doi: 10.1017/S0022215111002969. Epub 2011 Nov 4.

Abstract

Objective: To demonstrate that patients who have been intubated for prolonged periods of time will have an increased likelihood of developing bacterial biofilm on their endotracheal tubes.

Methods: We collected endotracheal tubes from patients at the time of extubation, and analysed representative sections with scanning electron microscopy for morphologic evidence of biofilms.

Results: From September 2007 to September 2008, 32 endotracheal tubes were analysed with electron microscopy. Patients who had been intubated for 6 days or longer had a significantly higher percentage of endotracheal tubes that exhibited bacterial biofilms, compared with patients intubated for less than 6 days (88.9 versus 57.1 per cent, p = 0.0439).

Conclusions: Longer duration of intubation is associated with a higher incidence of bacterial biofilm. Further research is needed to link the presence of bacterial biofilms to acquired laryngotracheal damage.

MeSH terms

  • Biofilms*
  • Equipment Contamination*
  • Humans
  • Intubation, Intratracheal / adverse effects
  • Intubation, Intratracheal / instrumentation*
  • Laryngeal Mucosa / microbiology
  • Laryngeal Mucosa / pathology
  • Laryngostenosis / etiology
  • Laryngostenosis / microbiology
  • Microscopy, Electron, Scanning
  • Pseudomonas / isolation & purification
  • Pseudomonas / physiology*
  • Staphylococcus aureus / isolation & purification
  • Staphylococcus aureus / physiology*
  • Time Factors
  • Trachea / microbiology
  • Tracheal Stenosis / etiology
  • Tracheal Stenosis / microbiology