A bienzymatic amperometric immunosensor exploiting supramolecular construction for ultrasensitive protein detection

Chem Commun (Camb). 2012 Jan 25;48(7):1045-7. doi: 10.1039/c2cc16177j. Epub 2011 Dec 8.

Abstract

Self-assembly of a ferrocene-appended polymer bearing an antigen fragment and lactate oxidase on a cyclodextrin-modified surface provides a highly sensitive, easy-to-operate and self-sufficient immunosensor.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / chemistry
  • Autoantibodies / analysis*
  • Biosensing Techniques / methods*
  • Cyclodextrins / chemistry
  • Electrochemical Techniques / methods
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism
  • Ferrous Compounds / chemistry
  • Humans
  • Immunoassay / methods*
  • Metallocenes
  • Mixed Function Oxygenases / chemistry
  • Mixed Function Oxygenases / metabolism
  • Sensitivity and Specificity
  • Surface Properties

Substances

  • Antigens
  • Autoantibodies
  • Cyclodextrins
  • Enzymes, Immobilized
  • Ferrous Compounds
  • Metallocenes
  • Mixed Function Oxygenases
  • lactate 2-monooxygenase
  • ferrocene