A novel Gram-stain-positive, aerobic, rod-shaped bacterium, designated strain D94-1(T), was isolated from soil collected in Liancheng county, Fujian province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain D94-1(T) was a member of the genus Nocardioides and was most closely related to Nocardioides salarius DSM 18239(T) (98.54% 16S rRNA gene sequence similarity), Nocardioides marinisabuli DSM 18965(T) (98.30%), Nocardioides basaltis KCTC 19365(T) (98.10%) and Nocardioides dokdonensis KCTC 19309(T) (97.76%). Phenotypic characteristics and DNA-DNA relatedness data served to distinguish strain D94-1(T) from N. salarius, N. marinisabuli, N. basaltis and N. dokdonensis. The chemotaxonomic properties of strain D94-1(T) were consistent with those of the genus Nocardioides: the cell-wall peptidoglycan type was based on ll-diaminopimelic acid, the predominant menaquinone was MK-8(H(4)) and the major fatty acids were iso-C(16:0) (29.15%) and anteiso-C(17:0) (21.0%). The DNA G+C content was 71.8 mol%. On the basis of the evidence presented in this study, strain D94-1(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides lianchengensis sp. nov. is proposed. The type strain is D94-1(T) (=DSM 24663(T)=CGMCC 4.6858(T)).