Culture confirmation of Listeria monocytogenes using tmRNA as a diagnostics target

Eoin Clancy; Barry Glynn; Kate Reddington; Terry Smith; Thomas Barry

doi:10.1016/j.mimet.2011.12.014

Culture confirmation of Listeria monocytogenes using tmRNA as a diagnostics target

J Microbiol Methods. 2012 Mar;88(3):427-9. doi: 10.1016/j.mimet.2011.12.014. Epub 2012 Jan 10.

Authors

Eoin Clancy¹, Barry Glynn, Kate Reddington, Terry Smith, Thomas Barry

Affiliation

¹ Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland.

PMID: 22261139
DOI: 10.1016/j.mimet.2011.12.014

Abstract

16s ribosomal RNA (rRNA) is routinely used to identify bacteria in direct detection culture confirmation assays. In some instances rRNA cannot be used as a target to distinguish between phylogenetically closely related bacteria. Here we evaluate an alternative target, transfer messenger RNA (tmRNA), for the culture confirmation of Listeria monocytogenes.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteriological Techniques / methods*
Listeria monocytogenes / classification*
Listeria monocytogenes / genetics*
Listeria monocytogenes / growth & development
Listeria monocytogenes / isolation & purification
Listeriosis / diagnosis
Listeriosis / microbiology*
Molecular Diagnostic Techniques / methods*
RNA, Bacterial / genetics*

Substances

RNA, Bacterial
tmRNA