Optimization of pulsed-field gel electrophoresis protocols for Salmonella Paratyphi A subtyping

Foodborne Pathog Dis. 2012 Apr;9(4):325-30. doi: 10.1089/fpd.2011.1023.

Abstract

Salmonella enterica serovar Paratyphi A infection has caused public health problems in some countries in recent years. Pulsed-field gel electrophoresis (PFGE) has been used for the subtyping and epidemiological investigations of some serotypes of Salmonella, mainly in outbreaks caused by non-typhoidal Salmonella. In this study, different restriction endonucleases and electrophoresis parameters were compared for the PFGE subtyping by using Salmonella Paratyphi A strain panels. Two protocols for the enzymes SpeI and XbaI showed higher discriminatory power, which may facilitate epidemiological analysis for more accurate case definition, and clonality study of Salmonella Paratyphi A.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • China / epidemiology
  • Cluster Analysis
  • DNA Restriction Enzymes
  • DNA, Bacterial / genetics
  • Deoxyribonucleases, Type II Site-Specific
  • Disease Outbreaks / classification*
  • Electrophoresis, Gel, Pulsed-Field / methods*
  • Electrophoresis, Gel, Pulsed-Field / standards
  • Paratyphoid Fever / epidemiology
  • Paratyphoid Fever / microbiology*
  • Pilot Projects
  • Public Health
  • Salmonella paratyphi A / classification*
  • Salmonella paratyphi A / genetics
  • Time Factors

Substances

  • DNA, Bacterial
  • DNA Restriction Enzymes
  • endodeoxyribonuclease SpeI
  • endodeoxyribonuclease XBAI
  • Deoxyribonucleases, Type II Site-Specific