Regeneration of comparative genomic hybridization oligonucleotide microarrays with dimethylurea

Anal Biochem. 2012 Jul 15;426(2):91-3. doi: 10.1016/j.ab.2012.04.013. Epub 2012 Apr 13.

Abstract

Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Comparative Genomic Hybridization*
  • DNA / metabolism
  • DNA Copy Number Variations
  • Humans
  • Methylurea Compounds / chemistry*
  • Oligonucleotide Array Sequence Analysis*

Substances

  • Methylurea Compounds
  • DNA
  • 1,3-dimethylurea