Abstract
A novel ATP-dependent DNA topoisomerase which makes reversible double-strand breaks in the DNA double helix has been purified to near homogeneity from T4 bacteriophage-infected Escherichia coli cells. Genetic data suggest that this activity is essential for initiating T4 DNA replication forks in vivo.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphate / metabolism
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DNA Replication*
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DNA Topoisomerases, Type I / isolation & purification
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DNA Topoisomerases, Type I / metabolism*
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DNA, Superhelical / metabolism
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Genes
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Genes, Viral
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Mutation
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RNA / metabolism
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T-Phages / enzymology
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T-Phages / genetics*
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Virus Replication*
Substances
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DNA, Superhelical
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RNA
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Adenosine Triphosphate
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DNA Topoisomerases, Type I