T4 DNA topoisomerase: a new ATP-dependent enzyme essential for initiation of T4 bacteriophage DNA replication

Nature. 1979 Oct 11;281(5731):456-61. doi: 10.1038/281456a0.

Abstract

A novel ATP-dependent DNA topoisomerase which makes reversible double-strand breaks in the DNA double helix has been purified to near homogeneity from T4 bacteriophage-infected Escherichia coli cells. Genetic data suggest that this activity is essential for initiating T4 DNA replication forks in vivo.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • DNA Replication*
  • DNA Topoisomerases, Type I / isolation & purification
  • DNA Topoisomerases, Type I / metabolism*
  • DNA, Superhelical / metabolism
  • Genes
  • Genes, Viral
  • Mutation
  • RNA / metabolism
  • T-Phages / enzymology
  • T-Phages / genetics*
  • Virus Replication*

Substances

  • DNA, Superhelical
  • RNA
  • Adenosine Triphosphate
  • DNA Topoisomerases, Type I