Abstract
The cellular receptor utilized by adenovirus serotype 26 (Ad26) has remained unclear. Here we show that Ad26 transduction is CD46-dependent and is efficiently blocked by anti-CD46 but not anti-CAR antibodies, demonstrating that Ad26 utilizes CD46 as a primary cellular receptor. Moreover, following Ad26 vaccination of rhesus monkeys, we did not observe sustained activation of peripheral or mucosal vector-specific CD4(+) T lymphocytes. These data contribute to our understanding of Ad26 as a candidate vaccine vector.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics*
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Adenoviridae / metabolism*
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Adenoviridae Infections / immunology
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Adenoviridae Infections / virology*
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Animals
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Antibodies, Monoclonal / chemistry
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Coxsackie and Adenovirus Receptor-Like Membrane Protein / chemistry*
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Coxsackie and Adenovirus Receptor-Like Membrane Protein / metabolism
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Genetic Vectors
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Green Fluorescent Proteins / metabolism
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Humans
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Inflammation
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Leukocytes, Mononuclear / virology
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Lymphocyte Activation*
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Macaca mulatta
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Membrane Cofactor Protein / chemistry*
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T-Lymphocytes / immunology
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T-Lymphocytes / virology*
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Vaccination
Substances
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Antibodies, Monoclonal
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Coxsackie and Adenovirus Receptor-Like Membrane Protein
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Membrane Cofactor Protein
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Green Fluorescent Proteins