Adenovirus serotype 26 utilizes CD46 as a primary cellular receptor and only transiently activates T lymphocytes following vaccination of rhesus monkeys

J Virol. 2012 Oct;86(19):10862-5. doi: 10.1128/JVI.00928-12. Epub 2012 Jul 18.

Abstract

The cellular receptor utilized by adenovirus serotype 26 (Ad26) has remained unclear. Here we show that Ad26 transduction is CD46-dependent and is efficiently blocked by anti-CD46 but not anti-CAR antibodies, demonstrating that Ad26 utilizes CD46 as a primary cellular receptor. Moreover, following Ad26 vaccination of rhesus monkeys, we did not observe sustained activation of peripheral or mucosal vector-specific CD4(+) T lymphocytes. These data contribute to our understanding of Ad26 as a candidate vaccine vector.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / metabolism*
  • Adenoviridae Infections / immunology
  • Adenoviridae Infections / virology*
  • Animals
  • Antibodies, Monoclonal / chemistry
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein / chemistry*
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein / metabolism
  • Genetic Vectors
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Inflammation
  • Leukocytes, Mononuclear / virology
  • Lymphocyte Activation*
  • Macaca mulatta
  • Membrane Cofactor Protein / chemistry*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / virology*
  • Vaccination

Substances

  • Antibodies, Monoclonal
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • Membrane Cofactor Protein
  • Green Fluorescent Proteins