Abstract
Based on sequence variation in the N-terminus of the UL55 gene, which encodes glycoprotein B (gB), human cytomegalovirus (CMV) can be classified into four gBn genotypes. We assessed the distribution of CMV gBn genotypes and the correlation between CMV gBn DNA (detected by real-time PCR) and CMV-positive pp65 cells (identified by immunohistochemical staining) in a cohort of hematopoietic stem cell transplant patients. The distribution of gB genotypes was as follows: gBn1, 60% of patients; gBn2, 13.3%; mixed gBn1 and gBn3 infection, 26.7%; and gBn4 and other mixed infections, 0%. CMV gBn1 was the most common genotype. The detected level of CMV gB DNA correlated well with the number of CMV-positive pp65 cells detected by immunostaining (r = 0.585).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Cytomegalovirus / genetics
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Cytomegalovirus / isolation & purification*
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Female
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Glycoproteins / genetics*
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Hematopoietic Stem Cell Transplantation*
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Humans
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Male
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Real-Time Polymerase Chain Reaction
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Viral Proteins / genetics*
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Young Adult
Substances
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Glycoproteins
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Viral Proteins
Grants and funding
This study was supported by the National Natural Science Foundation of China (30872239), Research of Health Department of China Province-Ministry Co-construct (WKJ2009-2-023), and the Technology Team for Major Infectious Diseases Prevention Control, Warning and Treatment (2009R50041). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.