Molecular cloning and sequence analysis of an extracellular protease from four Bacillus subtilis strains

Xujun Han; Yuh Shiwa; Masanori Itoh; Tohru Suzuki; Hirofumi Yoshikawa; Toshiyuki Nakagawa; Hiroko Nagano

doi:10.1271/bbb.120920

Molecular cloning and sequence analysis of an extracellular protease from four Bacillus subtilis strains

Biosci Biotechnol Biochem. 2013;77(4):870-3. doi: 10.1271/bbb.120920. Epub 2013 Apr 7.

Authors

Xujun Han¹, Yuh Shiwa, Masanori Itoh, Tohru Suzuki, Hirofumi Yoshikawa, Toshiyuki Nakagawa, Hiroko Nagano

Affiliation

¹ United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.

PMID: 23563549
DOI: 10.1271/bbb.120920

Abstract

Four Bacillus subtilis strains were isolated from traditional fermented foods, and the sequences of their extracellular alkaline proteases (AprE) were analyzed and cloned. The recombinant enzymes synthesized by means of Escherichia coli exhibited high proteolytic activity. AprE CN2 showed hydrolytic activity 4-fold higher than that of AprE 168. This activity was also seen in the presence of relatively high NaCl concentrations.

MeSH terms

Amino Acid Sequence
Bacillus subtilis / cytology
Bacillus subtilis / enzymology*
Bacillus subtilis / genetics*
Bacterial Proteins / chemistry
Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Cloning, Molecular
Endopeptidases / chemistry
Endopeptidases / genetics*
Endopeptidases / metabolism
Extracellular Space / enzymology*
Kinetics
Molecular Sequence Data
Sequence Analysis*
Sodium Chloride / pharmacology

Substances

Bacterial Proteins
Sodium Chloride
Endopeptidases
alkaline protease