We aim to assess the gender preferences for endoscopists among Hispanics and factors influencing such preferences. Cross-sectional study in prospectively enrolled Hispanic patients using a pre-endoscopy questionnaire regarding their gender preferences for the endoscopist and the reasons for such preferences. Multivariate logistic regression model was used for the statistical analysis. We enrolled total 200 Hispanic patients (100 males, 100 females) in our study. Their mean age was 51 ± 14 years. 30 % of Hispanics expressed a gender preference for the endoscopist. Gender preference was more common among Hispanic women than men (38 % vs. 22 %, p = 0.014). Gender preference for primary care provider was independently associated with the gender preference for the endoscopist (odds ratio 66, 95 % CI 25-182, p < 0.0001). We found significant number of Hispanic patients with gender preference for the endoscopist (female more than male). The odds of such preferences were strongly associated with the gender preference for the primary care physician.