NADPH:O2 oxidoreductase of human eosinophils in the cell-free system

B G Bolscher; L Koenderman; A T Tool; P M Stokman; D Roos

doi:10.1016/0014-5793(90)81025-j

NADPH:O2 oxidoreductase of human eosinophils in the cell-free system

FEBS Lett. 1990 Jul 30;268(1):269-73. doi: 10.1016/0014-5793(90)81025-j.

Authors

B G Bolscher¹, L Koenderman, A T Tool, P M Stokman, D Roos

Affiliation

¹ Central Laboratory, Netherlands Red Cross Blood Transfusion Service, Amsterdam.

PMID: 2384165
DOI: 10.1016/0014-5793(90)81025-j

Abstract

The NADPH oxidase of human eosinophils, measured in the cell-free system, shows the same characteristics as the enzyme from human neutrophils. All proteins required for activity of the enzyme are expressed in eosinophils at a higher level than in neutrophils. Eosinophils isolated from patients with chronic granulomatous disease show the same molecular defects as the neutrophils from these patients.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkaline Phosphatase / metabolism
Blotting, Western
Cell Membrane / enzymology
Cell-Free System
Enzyme Activation / drug effects
Eosinophils / enzymology*
Granulomatous Disease, Chronic / enzymology
Humans
NADH, NADPH Oxidoreductases / metabolism*
NADPH Oxidases
Neutrophils / enzymology
Sodium Dodecyl Sulfate / pharmacology

Substances

Sodium Dodecyl Sulfate
NADH, NADPH Oxidoreductases
NADPH Oxidases
Alkaline Phosphatase