Abstract
Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.
Keywords:
Influenza; neutralization tests; pseudovirus; serology; subtype H7N9; viruses.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Aged, 80 and over
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Antibodies, Neutralizing / blood
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Capsid Proteins / genetics
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Cloning, Molecular
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Genetic Engineering
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Genetic Vectors
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HEK293 Cells
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Humans
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Influenza A Virus, H7N9 Subtype / genetics
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Influenza A Virus, H7N9 Subtype / immunology*
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Influenza, Human / blood
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Influenza, Human / immunology*
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Influenza, Human / virology
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Lentivirus / genetics
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Middle Aged
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Neutralization Tests*
Substances
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Antibodies, Neutralizing
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Capsid Proteins