Safe pseudovirus-based assay for neutralization antibodies against influenza A(H7N9) virus

Emerg Infect Dis. 2013 Oct;19(10):1685-7. doi: 10.3201/eid1910.130728.

Abstract

Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus-based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.

Keywords: Influenza; neutralization tests; pseudovirus; serology; subtype H7N9; viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antibodies, Neutralizing / blood
  • Capsid Proteins / genetics
  • Cloning, Molecular
  • Genetic Engineering
  • Genetic Vectors
  • HEK293 Cells
  • Humans
  • Influenza A Virus, H7N9 Subtype / genetics
  • Influenza A Virus, H7N9 Subtype / immunology*
  • Influenza, Human / blood
  • Influenza, Human / immunology*
  • Influenza, Human / virology
  • Lentivirus / genetics
  • Middle Aged
  • Neutralization Tests*

Substances

  • Antibodies, Neutralizing
  • Capsid Proteins