Monitoring of patients supported by extracorporeal membrane oxygenation for systemic infections by broad-range rRNA gene PCR amplification and sequence analysis

J Clin Microbiol. 2014 Jan;52(1):307-11. doi: 10.1128/JCM.02493-13. Epub 2013 Oct 23.

Abstract

The rRNA gene PCR and sequencing test, SepsiTest, was compared with blood culture (BC) regarding the diagnosis of pathogens in 160 blood samples drawn from 28 patients during extracorporeal membrane oxygenation. With 45% of positive samples, SepsiTest was 13 to 75 h faster than BC. SepsiTest indicated bacteremias in 25% of patients who were BC negative.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Extracorporeal Membrane Oxygenation / adverse effects*
  • Female
  • Genes, rRNA / genetics*
  • Humans
  • Male
  • Microbiological Techniques / methods*
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Polymerase Chain Reaction / methods
  • Sepsis / diagnosis*
  • Sequence Analysis / methods
  • Time Factors
  • Young Adult