Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

Yuan Liu; Haohua Tu; Wladimir A Benalcazar; Eric J Chaney; Stephen A Boppart

doi:10.1109/JSTQE.2011.2168559

Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm

IEEE J Sel Top Quantum Electron. 2012 May;18(3):10.1109/JSTQE.2011.2168559. doi: 10.1109/JSTQE.2011.2168559.

Authors

Yuan Liu¹, Haohua Tu, Wladimir A Benalcazar, Eric J Chaney, Stephen A Boppart

Affiliation

¹ Department of Bioengineering, Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801 USA ( yuanliu3@illinois.edu ).

Abstract

Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.

Keywords: Biomedical imaging; biophotonics; nonlinear optics; optical microscopy; supercontinuum (SC) generation.

Abstract

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