Frequency analyses of active NORs in nuclei of artificially induced triploid fishes

M Flajšhans; P Ráb; S Dobosz

doi:10.1007/BF00223846

Frequency analyses of active NORs in nuclei of artificially induced triploid fishes

Theor Appl Genet. 1992 Oct;85(1):68-72. doi: 10.1007/BF00223846.

Authors

M Flajšhans¹, P Ráb, S Dobosz

Affiliation

¹ Department of Fish Genetics and Breeding, Research Institute of Fish Culture and Hydrobiology, CS-389 25, Vodňany, Czechoslovakia.

PMID: 24197230
DOI: 10.1007/BF00223846

Abstract

The correspondence between increased numbers of both chromosomal and nuclear NORs and artificially induced triploidy in three fish species (rainbow trout, Oncorhynchus mykiss; common carp, Cyprinus carpio; and tench, Tinea tinea) has been confirmed by CMA3 fluorescence and Ag-staining. The frequencies of cell nuclei with one, two and three active NORs, as revealed by Ag-staining, has been analyzed statistically to find the minimum cell number which verifies the increased ploidy level. A minimum sample size of about 80 cells exhibiting three active NORs is sufficient to confirm triploidy in all three species and may be of use for categorising other ploidy-manipulated fish species.