Protamine-adsorbed magnetic nanoparticles for efficient isolation and concentration of hepatitis-C virus from human plasma samples

Chem Commun (Camb). 2014 Jan 18;50(5):590-2. doi: 10.1039/c3cc46793g. Epub 2013 Nov 25.

Abstract

Protamine hydrochloride adsorbed onto citrated superparamagnetic iron oxide nanoparticles represents an efficient tool for capturing and concentration of hepatitis-C virus from plasma samples, improving the sensitivity of downstream analysis by nucleic acid testing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Dextrans / chemistry
  • Hepacivirus / chemistry*
  • Hepacivirus / genetics
  • Hepacivirus / isolation & purification
  • Humans
  • Magnetite Nanoparticles / chemistry*
  • Protamines / chemistry*
  • RNA, Viral / analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Thrombin / chemistry
  • Thrombin / metabolism

Substances

  • Dextrans
  • Magnetite Nanoparticles
  • Protamines
  • RNA, Viral
  • Thrombin
  • ferumoxides