Use of IRF-3 and/or IRF-7 knockout mice to study viral pathogenesis: lessons from a murine retrovirus-induced AIDS model

J Virol. 2014 Feb;88(4):2349-53. doi: 10.1128/JVI.02960-13. Epub 2013 Dec 11.

Abstract

Interferon regulatory factor (IRF) regulation of the type I interferon response has not been extensively explored in murine retroviral infections. IRF-3(-/-) and select IRF-3/7(-/-) mice were resistant to LP-BM5-induced pathogenesis. However, further analyses strongly suggested that resistance could be attributed to strain 129-specific contamination of the known retrovirus resistance gene Fv1. Therefore, caution should be taken when interpreting phenotypes observed in these knockout mice, as strain 129-derived genetic polymorphisms may explain observed differences.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal*
  • Electrophoresis
  • Gammaretrovirus / genetics
  • Gammaretrovirus / immunology*
  • Interferon Regulatory Factor-3 / genetics*
  • Interferon Regulatory Factor-7 / genetics*
  • Interferon Type I / immunology*
  • Mice
  • Mice, Knockout
  • Murine Acquired Immunodeficiency Syndrome / immunology*
  • Murine Acquired Immunodeficiency Syndrome / physiopathology*
  • Murine Acquired Immunodeficiency Syndrome / virology
  • Proteins / genetics
  • Proteins / immunology
  • Species Specificity
  • Statistics, Nonparametric

Substances

  • Fv1 protein, mouse
  • Interferon Regulatory Factor-3
  • Interferon Regulatory Factor-7
  • Interferon Type I
  • Proteins