Detection of dehalogenation impurities in organohalogenated pharmaceuticals by UHPLC-DAD-HRESIMS

J Pharm Biomed Anal. 2014 Apr:92:1-5. doi: 10.1016/j.jpba.2013.12.043. Epub 2014 Jan 7.

Abstract

The presence of dehalogenated impurities is often observed in halogen-containing pharmaceuticals, and can present a difficult analytical challenge, as the chromatographic behavior of the halogenated drug and the hydrogen-containing analog can be quite similar. In this study we describe the chromatographic separation and unambiguous identification of dehalogenation impurities or associated isomers in organohalogenated pharmaceuticals using UHPLC with a pentafluorophenyl column coupled with diode-array and high resolution electrospray ionization mass spectrometry detection (UHPLC-DAD-HRESIMS).

Keywords: Dehalogenation impurities; HRMS; Method development; Perfluorophenyl; UHPLC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Drug Contamination
  • Halogenation
  • Halogens / chemistry*
  • Pharmaceutical Preparations / chemistry*
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Halogens
  • Pharmaceutical Preparations