Influence of factor 5 rs6025 and factor 2 rs1799963 mutation on inhibitor development in patients with hemophilia A--an Israeli-German multicenter database study

Thromb Res. 2014 Apr;133(4):544-9. doi: 10.1016/j.thromres.2014.01.005. Epub 2014 Jan 17.

Abstract

Objective: The present cohort study was performed to investigate the impact of the factor 5 rs6025 [F5] and the factor 2 rs1799963 [F2] mutations on high-titer inhibitor development [HRI] in patients with severe/moderate-severe hemophilia A [HA].

Patients and methods: 216 patients with F8<2% born between 1980 and 2011 were followed after initial HA diagnosis over the first 200 exposure days. The first HA patient per family who presented for diagnosis was included in the present study.

Results: 32 of 216 children [14.8%] tested for F5/F2 carried either the F5 or the F2 variant. HRI occurred in 14 out of 32F5/F2-carriers compared with 40 of 184 without F5/F2. Multivariate analysis adjusted for F8 genotype, treatment intensity, first-line use of plasma derived FVIII versus recombinant FVIII concentrates revealed that the presence of F5/F2 independently increases the risk of HRI development to odds [OR] of 3.4. Large deletions in the F8 gene [OR: 5.10], patients from Israel [OR: 4.0], the increase of FVIII per one IU/kgbw [OR: 1.05] and birth year [OR: 1.12] were significantly associated with the risk to develop HRI.

Conclusion: Data presented here suggest that HRI development is of multifactorial origin and that F5 and F2 mutations may contribute to this risk.

Keywords: F2 rs1799963 mutation; F5 rs6025; Hemophilia A; High-titer inhibitor development.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Autoantibodies / biosynthesis
  • Autoantibodies / immunology
  • Child
  • Child, Preschool
  • Cohort Studies
  • Factor V / genetics*
  • Factor VIII / antagonists & inhibitors
  • Factor VIII / immunology
  • Factor VIII / metabolism
  • Factor VIII / therapeutic use
  • Female
  • Genotype
  • Germany
  • Hemophilia A / drug therapy
  • Hemophilia A / genetics*
  • Hemophilia A / immunology
  • Hemophilia A / metabolism
  • Humans
  • Israel
  • Male
  • Mutation*
  • Risk Factors
  • Young Adult

Substances

  • Autoantibodies
  • Factor V
  • Factor VIII