Photosensitizer loaded nano-graphene for multimodality imaging guided tumor photodynamic therapy

Pengfei Rong; Kai Yang; Avinash Srivastan; Dale O Kiesewetter; Xuyi Yue; Fu Wang; Liming Nie; Ashwinkumar Bhirde; Zhe Wang; Zhuang Liu; Gang Niu; Wei Wang; Xiaoyuan Chen

doi:10.7150/thno.8070

Photosensitizer loaded nano-graphene for multimodality imaging guided tumor photodynamic therapy

Theranostics. 2014 Jan 15;4(3):229-39. doi: 10.7150/thno.8070. eCollection 2014.

Authors

Pengfei Rong¹, Kai Yang², Avinash Srivastan³, Dale O Kiesewetter³, Xuyi Yue⁴, Fu Wang³, Liming Nie⁴, Ashwinkumar Bhirde³, Zhe Wang³, Zhuang Liu⁵, Gang Niu³, Wei Wang⁶, Xiaoyuan Chen³

Affiliations

¹ 1. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China ; 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 3. State Key Laboratory for Powder Metallurgy, Central South University, Changsha, Hunan, 410083, China.
² 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 4. Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu 215123, China.
³ 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States.
⁴ 2. Laboratory of Molecular Imaging and Nanomedicine (LOMIN), National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health (NIH), Bethesda, Maryland 20892, United States ; 5. Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen, Fujian 361102, China.
⁵ 4. Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou, Jiangsu 215123, China.
⁶ 1. Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China.

Abstract

Graphene, a 2-dimensional carbon nanomaterial, has attracted wide attention in biomedical applications, owing to its intrinsic physical and chemical properties. In this work, a photosensitizer molecule, 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-alpha (HPPH or Photochlor®), is loaded onto polyethylene glycol (PEG)-functionalized graphene oxide (GO) via supramolecular π-π stacking. The obtained GO-PEG-HPPH complex shows high HPPH loading efficiency. The in vivo distribution and delivery were tracked by fluorescence imaging as well as positron emission tomography (PET) after radiolabeling of HPPH with (64)Cu. Compared with free HPPH, GO-PEG-HPPH offers dramatically improved photodynamic cancer cell killing efficacy due to the increased tumor delivery of HPPH. Our study identifies a role for graphene as a carrier of PDT agents to improve PDT efficacy and increase long-term survival following treatment.

Keywords: Graphene oxide; HPPH; optical imaging.; photodynamic therapy; positron emission tomography.

Publication types

Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line, Tumor
Cell Survival
Chlorophyll / analogs & derivatives
Chlorophyll / chemistry
Chlorophyll / therapeutic use
Copper Radioisotopes / therapeutic use
Graphite / chemistry*
Mice
Microscopy, Fluorescence
Multimodal Imaging
Nanoparticles / chemistry*
Nanoparticles / therapeutic use
Neoplasms / drug therapy*
Photochemotherapy / methods*
Photosensitizing Agents / chemical synthesis
Photosensitizing Agents / therapeutic use*
Polyethylene Glycols / chemistry
Positron-Emission Tomography*

Substances

Copper Radioisotopes
Photosensitizing Agents
Chlorophyll
2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-a
Polyethylene Glycols
Graphite

Grants and funding

Intramural NIH HHS/United States