Eliminating acute lymphoblastic leukemia cells from human testicular cell cultures: a pilot study

Abstract

Objective: To study whether acute lymphoblastic leukemia (ALL) cells survive in a human testicular cell culture system.

Design: Experimental laboratory study.

Setting: Reproductive biology laboratory, academic medical center.

Patient(s): Acute lymphoblastic leukemia cells from three patients and testicular cells from three other patients.

Intervention(s): Acute lymphoblastic leukemia cells were cultured alone or in combination with testicular cells, at various concentrations, in a system that has recently been developed to propagate human spermatogonial stem cells.

Main outcome measure(s): Viability of ALL and testicular cells during culture was evaluated by flow cytometry using markers for live/dead cells. Furthermore, the presence of ALL cells among testicular cells was determined by highly sensitive (1:10,000 to 1:100,000 cells) patient-specific antigen-receptor minimal residual disease polymerase chain reaction. The presence of spermatogonia at the end of culture was determined by reverse transcription-polymerase chain reaction for ZBTB16, UCHL1, and GPR125.

Result(s): The ALL cells cultured separately did not survive beyond 14 days of culture. When cultured together with testicular cells, even at extremely high initial concentrations (40% ALL cells), ALL cells were undetectable beyond 26 days of culture. Reverse transcription-polymerase chain reaction confirmed the presence of spermatogonia at the end of the culture period.

Conclusion(s): Our pilot study shows that the described testicular cell culture system not only allows for efficient propagation of spermatogonial stem cells but also eliminates contaminating ALL cells.

Keywords: Fertility preservation; childhood cancer; human testis; leukemia; spermatogonial stem cell.