Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.
Keywords: H7N9; NA 292K mutant virus; R292K wild-type virus; SNP; influenza; influenza A(H7N9) virus; influenza virus; mutation; neuraminidase; oseltamivir resistance; real-time RT-PCR; real-time reverse transcription PCR; single-nucleotide polymorphism; viruses.