PCR for detection of oseltamivir resistance mutation in influenza A(H7N9) virus

Emerg Infect Dis. 2014 May;20(5):847-9. doi: 10.3201/eid2005.131364.

Abstract

Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.

Keywords: H7N9; NA 292K mutant virus; R292K wild-type virus; SNP; influenza; influenza A(H7N9) virus; influenza virus; mutation; neuraminidase; oseltamivir resistance; real-time RT-PCR; real-time reverse transcription PCR; single-nucleotide polymorphism; viruses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antiviral Agents / pharmacology*
  • Drug Resistance, Viral / genetics*
  • Humans
  • Influenza A Virus, H7N9 Subtype / drug effects*
  • Influenza A Virus, H7N9 Subtype / genetics*
  • Influenza, Human / drug therapy
  • Influenza, Human / epidemiology
  • Influenza, Human / virology
  • Mutation*
  • Neuraminidase / genetics
  • Oseltamivir / pharmacology*
  • Polymerase Chain Reaction
  • Viral Proteins / genetics

Substances

  • Antiviral Agents
  • Viral Proteins
  • Oseltamivir
  • NA protein, influenza A virus
  • Neuraminidase