Interleukin-6 and interleukin-1 production in acute leukemia with monocytoid differentiation

Blood. 1989 Nov 1;74(6):2081-7.

Abstract

Several authors have reported the in vitro production of colony-stimulating factors (CSF) and interleukin-1 (IL-1) by the neoplastic cells from patients with acute myeloid leukemia (AML). Using a sensitive bioassay for IL-6, the capacity of the leukemic cells of 30 patients with AML to produce IL-6 was examined. IL-6 production was found to be specific for cells from patients with an AML with monocytic differentiation (12 of 15 M4 and M5 patients, 0 of 15 M1 and M2 patients). Moreover, IL-6 production was paralleled by IL-1 production. The IL-6- and IL-1-producing cells were mainly found in the more mature monocytic cell fractions, defined as CD14-positive and CD34-negative adherent cells. By limiting dilution experiments, it could be excluded that the production of IL-1 or IL-6 was due to contamination with normal monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34
  • Antigens, Differentiation
  • Antigens, Differentiation, Myelomonocytic
  • Blotting, Northern
  • Cell Adhesion
  • Cell Differentiation
  • Humans
  • In Vitro Techniques
  • Interleukin-1 / biosynthesis*
  • Interleukin-6 / biosynthesis*
  • Interleukin-6 / genetics
  • Leukemia, Monocytic, Acute / metabolism*
  • Leukemia, Myelomonocytic, Acute / metabolism*
  • Lipopolysaccharide Receptors
  • RNA, Messenger / genetics

Substances

  • Antigens, CD34
  • Antigens, Differentiation
  • Antigens, Differentiation, Myelomonocytic
  • Interleukin-1
  • Interleukin-6
  • Lipopolysaccharide Receptors
  • RNA, Messenger