Evaluation of bio-rad geenius HIV-1 and -2 assay as a confirmatory assay for detection of HIV-1 and -2 antibodies

Anne Hawthorne Hallen; Agneta Samuelson; Margareta Nordin; Jan Albert; Gordana Bogdanovic

doi:10.1128/CVI.00153-14

Evaluation of bio-rad geenius HIV-1 and -2 assay as a confirmatory assay for detection of HIV-1 and -2 antibodies

Clin Vaccine Immunol. 2014 Aug;21(8):1192-4. doi: 10.1128/CVI.00153-14. Epub 2014 Jun 18.

Authors

Anne Hawthorne Hallen¹, Agneta Samuelson², Margareta Nordin², Jan Albert³, Gordana Bogdanovic⁴

Affiliations

¹ Department of Clinical Microbiology, Vastmanland Hospital, Västerås, Sweden.
² Department of Clinical Microbiology, Karolinska University Hospital Stockholm, Sweden.
³ Department of Clinical Microbiology, Karolinska University Hospital Stockholm, Sweden Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.
⁴ Department of Clinical Microbiology, Karolinska University Hospital Stockholm, Sweden gordana.bogdanovic@karolinska.se.

Abstract

The performance of the new Bio-Rad Geenius HIV-1/2 confirmatory assay was compared with that of the Chiron RIBA HIV-1/HIV-2 strip immunoblot assay using 166 samples from HIV-1-, HIV-2-, or HIV-1/2-positive and HIV-negative individuals and two quality control panels. Our results indicate that the Geenius assay is a suitable alternative for confirmatory HIV testing.

MeSH terms

HIV Antibodies / blood*
HIV Infections / blood
HIV Infections / diagnosis*
HIV-1 / immunology
HIV-2 / immunology
Humans
Retrospective Studies
Sensitivity and Specificity
Serologic Tests / methods

Substances

HIV Antibodies